Illumina Bead Station
BeadArray Technology : A novel approach to microarrays
Illumina's BeadArray Technology is based on 3-micron silica beads that self assemble in microwells on either of two substrates: fiber optic bundles or planar silica slides. When randomly assembled on one of these two substrates, the beads have a uniform spacing of ~5.7 microns. Each bead is covered with hundreds of thousands of copies of a specific oligonucleotide that act as the capture sequences in one of Illumina's assays.
BeadArray Technology Highlights
- 100% array quality control: The manufacturing process includes a functional validation sequential hybridization of every single array element (bead with oligos). This process, called decoding, allows us to QC every feature of every array to ensure that each array element is present and functional.
- High feature redundancy: Each element is represented in high redundancy (> 20-fold), providing industry-leading data quality and reproducibility, through comparison and averaging of the many beads of the same type in an experiment.
- Inherent flexibility: New bead pools are easily synthesized and deployed onto either of our two substrates, providing rapid availability of new products and fast turnaround for custom panels.
Multi-sample array formats
BeadArray technology is deployed on either of two multi-sample array formats for DNA or RNA-analysis applications. With both the 96-sample Array Matrix and the multi-sample BeadChip formats, uniform pits are etched into the surface of each substrate to a depth of approximately 3 microns prior to assembly. Beads are then randomly assembled and held in these microwells by Van der Waals forces and hydrostatic interactions with the walls of the well.
Illumina Bead chips require 50-500ng of total RNA in a maximum volume of 11 uL.
See price list for all the available platforms.
For more details about the Illumina platform, please visit their web site.