|Equipment||Dartmouth*||Outside (academic)**||Outside(for profit)|
|ELISPOT Reader (Zeiss)||$25/h||$50/h||$75/h|
|Bio-Plex Array Reader||$50/h||$100/h||$150/h|
|Services||Dartmouth*||Outside (academic)**||Outside (for profit)|
|200 ml blood bag||$500||$1000||$1500|
|50 ml blood||to come||to come||to come|
|Plasma isolation/cryopreservation Serum isolation/cryopreservation|
|10 ml blood||$45||$90||$135|
|ELISPOT reading (per plate)||$40||$60||$120|
|Robosep cell subset enrichment (per sample)||$40||$60||$120|
|ELISPOT assay||to come||to come||to come|
|Cell subset phenotyping||to come||to come||to come|
When sorting on the FACStar Plus or Aria, we add one (for a non-sterile sort) or two (for a sterile sort) extra hours to account for instrument set-up time before sorting.
Analysis time is free. The lab has a computing area with Macintosh workstations. If you want to use a computer attached to a cytometer, acquisition of new data on the instruments has priority. For this reason, analysis time on computers attached to the instruments cannot be reserved in advance.
Booking schedules are on-line in CCOPS. Users are charged for time used and for time booked (even if it has not been used). Cancelled time will be charged unless it is cancelled 24 hours in advance and the cancellation has been confirmed with lab personnel. Users of the FACSAria or FACStar Plus need to confirm with Gary Ward about his availability for operation of the instrument before booking.
The sign-up schedule for the instruments is used (i) to reserve time, (ii) as accounting records for billing purposes, and (iii) for communication between users (e.g. Do I need to shut down the instrument or is someone following me at midnight? Will someone have five minutes to spare sometime during the day?). It is expected that users will be trained to run the flow cytometers and will then use those instruments without direct supervision. Training sessions and help for new users are provided free of charge.
If a user would like Shared Resource staff to run samples on these instruments, there will be an additional fee of $40/h.
The lab welcomes the opportunity to collaborate in development of new methodologies; there is no charge for development work or feasibility studies.
* Use of the Shared Resource is subsidized by the NCI Cancer Center Core Grant. This subsidy amounts to approximately one third of our actual running costs.
** Outside academic rates are based on 1.62 times (for college overheads) the estimated actual running costs (without Cancer Center subsidy).