Shared Resources

 

 

Services

Phase 1: Free Experimental Design

  • Biological questions
  • Biological replicates
  • Maximize statistical power
  • Feasibility
  • Budget

Phase 2: Data Generation

  • RNA/DNA Isolation
  • Sample QC
  • Microarrays, Affymetrix and Illumina bead
  • Deep Sequencing, Ion Torrent in house, Illumina via collaborators

Phase 3: Data Analysis and Validation (in association with Bioinformatics and Molecular Biology Cores)

  • Statistics, Clustering
  • PCR validation
  • Biological relevance

Quality Control for RNA and DNA

  • NanoDrop. Fiber optic system. No cuvettes. Very accurate determination of concentration and purity on A260/280 readings
  • Agilent Bioanalyzer. Very accurate determination of RNA quality (intactness)
Microarrays and Deep Sequencing
Mircoarrays
  • mRna levels
  • small RNA levels
  • Genomic in-dels from frozen and FFPE samples
  • Genomics DNA methylation levels
  • Genotyping
Deep Sequencing
  • DNA Sequencing: Single-read and long- (mate-pair) and short-insert (paired-end) reads for small whole-genome sequencing and re-sequencing, de novo sequencing, structural variation, and copy number analysis
  • Transcriptome analysis: Characterize mRNA splice variants, mRNAs, nuclear RNA, coding SNPSs, and relative expression of transcripts in one experiment; profile rare and discover novel RNAs absolute quantitative transcript data
  • Gene Regulation & Control Analysis: Study epigenetic modifications and DNA-protein interactions across the entire genome and obtain a comprehensive view of the epigenome