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Equipment

ZEISS LSM 510 META laser point scanning confocal microscope

ZEISS LSM 510 META laser point scanning confocal microscope

7 laser lines from four different lasers

  • 405 nm (DAPI, Hoechst, PPIX, Filipin, paGFP, Fluoro Gold)
  • Tunable Argon 458/477/488/514 (GFP, CFP, FITC, Alexa 488, YFP)
  • Hene 543 nm (Rhodamine, TxRed, Alexa 555, Propidium Iodide)
  • Hene 633 nm (Alexa 647, CY5, APC)

2 PMT light detectors (photomultiplier tubes)

1 Meta detector (spectral detector, can also be used as third PMT)

Photo-diode detector for transmitted-light imaging.

Axiovert 200 wide-field inverted fluorescence microscope.

Objectives available:

  • 5X Plan-Apochromat/0.16 NA
  • 10X Plan-Apo/0.45 NA
  • 20X Plan-Apo/0.75 NA with DIC capability
  • 40X Plan-Neo/1.3 NA Oil with DIC capability
  • 40X C-Apo/1.2 NA Water Immersion
  • 63X Plan-Apo/1.4 NA Oil with DIC capability
  • P stage insert for 37ºC work
  • Objective heater band for 63X Plan-Apo objective
Olympus IX-83 TIRF microscope

  • Zero Drift focus module
  • 406 nm, 491 nm, 561 nm, and 640 nm TIRF lasers
  • Prior Lumen 200 widefield fluorescence light source
  • Andor Zyla scMOS cameras and  Andor TuCam adapter
  • 60X 1.49 ApoN TIRF objective
  • 100X 1.4 UplanSApo non TIRF objective
  • Tokai HIT CO₂stage incubator
  • Computer and MetaMorph  Advanced software V 7.8.3.0
Olympus IX-73 Inverted Fluorescence Microscope with Olympus DP73 computer controlled CCD camera

Olympus IX-73 Inverted Fluorescence Microscope with Olympus DP73 computer controlled CCD camera

This is a new inverted fluorescence microscope system that allows imaging of multi-well plastic culture plates using long working distance 20X and 40X objectives. High quality imaging of coverslip covered samples can be done using the 20X and 40X dry objectives or with oil immersion 40X and 100X high NA PlanApo objectives. The microscope has a wide array of fluorescence filter cubes in the eight position turret to allow use of fluorophores such as DAPI, GFP, TxRed, mCherry, Cy5, and IR Dye 800.

Objectives available:

  • UPLFLN4X; U PLAN FLUORITE 4X OBJECTIVE,NA 0.13, WD 17MM
  • UPLSAPO10X2; U PLANS S-APO 10X OBJECTIVE; NA 0.4, WD 3.1MM
  • LUCPLFLN20XPH; LWD U PLAN FL PHI OBJ,NA 0.45,WD6.4-7.6MM,W/CC
  • LUCPLFLN40XPH; LWD U PLAN FL PH 2 OBJ,NA0.6,WD2.7-4.0,W/C-COL
  • UPLFLN40XO; U PLAN FLUORITE 40X OIL OBJ,NA 1.30,WD 0.2MM
  • UPLSAPO100XO; U PLAN S-APO 100X OIL OBJ,NA1.40,WD0.12

Fluorescence filter cubes available (8 positions):

  • DAPI-5060C-OFF-ZERO;EX377/50,EM447/60,DM409
  • TXRED-4040C-OFF-ZERO; EX562/40,EM624/40,DM593
  • GFP-3035D-OFF-ZERO; EX472/30,EM520/35,DM495
  • ET Cy5 longpass, EX620/60, EM660lp, DM660lpxrxt
  • ET LI-Cor for IR Dye 800, EX740/40, EM780lp, DM 770lpxr
  • ET DAPI/FITC/Texas Red
  • ET EGFP/mCherry
Olympus BX 50 upright fluorescence microscope

Olympus BX 50 upright fluorescence microscope

Objective available:

  • UPlanFL 10X/0.3
  • UPlanFL 20X/0.5
  • UPlanFL 40X/0.75
  • Zeiss Achroplan 100X/1,25 oil can be placed into the turret on request.

Fluorescence filter cubes:

  • DAPI:ex-BP360-370, DM400,em-BA420
  • FITC:ex-460-490, DM505, em-BA515IF
  • Rhodamine:ex-BP520-550, DM565, em-BA580IF
Zeiss M2Bio Stereomicroscope

Zeiss M2Bio Stereomicroscope

This stereomicroscope system provides low magnification long working distance (stereo) imaging with an 11X zoom. Field widths as large as 2.5 cm can be imaged. The microscope converts to monocular imaging with 10X or 20X LWD objectives and the 11X zoom. Trans or reflected Bright field and fluorescence imaging is possible. Images are recorded with either a B/W or color digital camera using Zeiss Axiovision software.

Objectives available:

  • 0.6X
  • 1.0X
  • 1.6X
  • 10X 0.45 NA f=200
  • 20X 0.6 NA f=200

Fluorescence Cubes available:

  • Q dot 705 ex 460SPUVv2; bs 475DCXRU; em D705/20
  • Coumarin ex D405/10, bs 425DCLP; em D460/50
  • cGFP ex D436/20; bs 455DCLP; em D480/40
  • GFP ex HQ470/40; bs Q495LP; em HQ525/50
  • yGFP ex HQ500/20; bs Q515LP; em HQ535/30
  • TRITC ex D540/25; bs 565DCLP; em D605/55
  • Cy5 ex HQ620/60; bs Q660LP; em HQ700/75
CCD digital camera

Olympus DP73, 17MP COLOR CAMERA, PIXEL SHIFTING COOLED