Services
- Provide consultation on transgenic mice
construct design and production, overall experimental design, utilization, maintenancebreeding and preservation - Genetic construct generation
Use recombineering in E. coli or yeast to generate constructs for experimental use - Oocyte injection transgenic production
Generate transgenic founders by injection of plasmids, BAC or lentivirus into oocytes and transplant the oocytes in foster mothers - ES cell culture for gene targeting or other purposes
- Provide a variety of ES cell lines, transfect with KO constructs, select clones with drug selection, freeze and provide lysates for genotyping,
expand and refreeze targeted clones for mouse generation, karyotype clones to select best clones - Generation of chimeric mice from ES cells
Inject ES cells into blastocysts to generate germline chimeric mice - Establish lines from frozen embryos from another institutions or rederive a pathogen infected transgenic line by embryo transfer
- Genotyping
Purify DNA from ES clones (to screen for targeted clones) or tail biopsies (to screen for founders or transgenic offspring). Establish a genotype assay if not already established.Genotype by PCR or Southern blot - Cigarette Smoke Exposure Analytical Lab
Expose rodents to tobacco smoke in the Teague smoke exposure facility - Cryopreservation of embryos and sperm
To preserve & protect your valuable transgenic mouse lines - Humanized mice
Creating specialized mice with a human immune system. Currently offering two different techniques using Nod/SCIDIL2 gamma receptor XO mice. - Other services
Tail biopsies, tail vein injections, ovariectomies, vasectomies, embryo transfer, blood drawing, timed pregnancies, superovulations, maintain transgenic colonies (husbandry), maintaining Flp and Cre expressing mice for mating to transgenic
Facilities
TGCSR currently maintains two specialized transgenic lines that express Cre or Flp in the germline for genetic manipulation of transgene/knockouts in vivo. TGCSR has a fully equipped embryo manipulation laboratory in the Borwell animal facility and an adjoining 4 room suite of mouse husbandry rooms.
