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Norris Cotton Cancer Center
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Microscopy Equipment

ZEISS LSM 800 META laser point scanning confocal microscope with Airyscan enhanced resolution

ZEISS LSM 800 laser point scanning confocal microscope with Airyscan enhanced resolution

Four Diode lasers for Confocal Imaging:

  • Diode laser (405 nm, 5 mW); class 3B (DAPI, Hoechst, PPIX, Filipin, paGFP)
  • Diode laser (488 nm, 10 mW); class 3B (GFP, CFP, FITC, AF488, YFP)
  • Diode (SHG) laser (561 nm, 10 mW); class 3B (Cy3, TxRed, AF568, AF594)
  • Diode laser (640 nm, 5 mW); laser class 3B (AF647, CY5, APC)

Confocal Detectors:

  • Two Gallium Arsenide Phosphide (GaAsP) PMTs
  • One additional GaAsP Airyscan enhanced resolution PMT detector for 63× objective

Lumencor LED Spectra X Light Engine- for Wide-field Fluorescence Microscopy:

  • Excitation 395/25
  • Excitation 470/24
  • Excitation 550/15
  • Excitation 640/30
  • Excitation 720/20

Wide-Field Bright-field and Fluorescence Detector:

  • Hamamatsu C11440-22CU Digital CMOS camera ORCA-Flash4.0

Electronically switchable illumination and detection module (ESID) for transmitted-light imaging.

Zeiss AxioObserver wide-field inverted fluorescence microscope

ZEISS AxioObserver wide-field inverted flourescence microscopeObjectives available:

  • 10X Plan-Apo/0.45 NA
  • 20X Plan-Apo/0.8 NA with DIC capability
  • 40X Plan-Apo/1.3 NA Oil with DIC capability
  • 40X C-Apo/1.2 NA Water Immersion
  • 63X Plan-Apo/1.4 NA Oil with DIC capability

Live Cell Incubation System

  • Full plexiglass enclosure, heating stage, and enclosure heater for 37ºC live imaging
  • CO2 control system
  • Definite Focus 2 autofocus

PerkinElmer Vectra® 3 automated quantitative pathology imaging system

This slide scanner and associated software for either immunofluorescence (IF) or immunohistochemistry (IHC) slides is located in Borwell 338 West. The Olympus BX51 upright fluorescence microscope is equipped with an automated 6-slide stage. The imaging system allows imaging and measurement of weakly expressing and overlapping biomarkers within a single H&E, IHC or IF intact thin tissue sections or tissue micro-arrays. Vectra, Phenochart, and inForm software allow control of multiplexed biomarker imaging and quantitative analysis.

Tissue thin sections or TMAs can be labeled with IF or IHC stains, or with conventional stains such as H&E and trichrome. When using IF or IHC stains, multiple proteins can be measured on a per tissue, per cell, or per cell compartment (e.g., nuclear, cytoplasmic) basis. Multi-spectral imaging using the Nuance camera allows unmixing of highly overlapping spectral signals and correction for autofluorescence.

Phenochart software permits contextual viewing and annotation capability of whole slide scans created using the 4X or 10X objectives. Composite images are automatically generated from the series of tiled images covering the slide coverslip area. Users can navigate around the stored digital image and identify areas of interest for further higher-resolution multispectral image acquisition using 20X or 40X objectives.

Objectives:

  • 4X   UPlan SApo NA 0.16 WD 13.0 mm
  • 10X UPlan SApo NA 0.40 WD   3.1 mm
  • 20X UPlan SApo NA 0.75 WD   0.6 mm
  • 40X UPlan SApo NA 0.95 WD   0.18 mm

Essen BioScience IncuCyte® ZOOM real-time, quantitative live-cell analysis system

Essen BioScience IncuCyte ZOOM real-time, quantitative live-cell analysis system

The IncuCyte Zoom is an automated microscope/camera system that remains inside our Panasonic CO2 incubator and allows non-perturbing imaging over extended periods of time. Long-term time course experiments can be performed using up to six multi-well plates. Quantification of cell proliferation, chemotaxis, or apoptosis is possible using integrated system software.

  • Objectives: 4X / 10X / 20X –manually switched
  • Image Resolution:
    • 4X: 3.05 um/pixel
    • 10X: 1.22 um/pixel
    • 20X: 0.61 um/pixel
  • Exported Image Formats: JPEG, PNG, TIFF, RAW
  • Exported Movie Formats: WMV, AVI
  • Fluorescence Excitation/Emission: LED Dual Color Module 4459
    Green 440-840 nm 504-544 nm
    Red 565-605 nm 625-705 nm
    Channel Excitation Emission
  • Bright-field Phase Contrast Imaging

Olympus IX-83 TIRF microscope

Olympus IX-83 TIRF microscope

  • Zero Drift focus module
  • 406 nm, 491 nm, 561 nm, and 640 nm TIRF lasers
  • Prior Lumen 200 widefield fluorescence light source
  • Andor Zyla scMOS cameras and Andor TuCam adapter
  • 60X 1.49 ApoN TIRF objective
  • 100X 1.4 UplanSApo non TIRF objective
  • Tokai HIT CO₂stage incubator
  • Dell Precision T5600 Computer and Olympus celSens Dimension V 1.16 software

Olympus IX-73 Inverted Fluorescence Microscope with Olympus DP73 computer controlled CCD camera

Olympus IX-73 Inverted Fluorescence Microscope with Olympus DP73 computer controlled CCD camera

This inverted fluorescence microscope system allows imaging of multi-well plastic culture plates using long working distance 20X and 40X objectives. High quality imaging of coverslip covered samples can be done using the 20X and 40X dry objectives or with oil immersion 40X and 100X high NA PlanApo objectives. The microscope has a wide array of fluorescence filter cubes in the eight position turret to allow use of fluorophores such as DAPI, GFP, TxRed, mCherry, Cy5, and IR Dye 800.

Objectives available:

  • UPLFLN4X; U PLAN FLUORITE 4X OBJECTIVE,NA 0.13, WD 17MM
  • UPLSAPO10X2; U PLANS S-APO 10X OBJECTIVE; NA 0.4, WD 3.1MM
  • LUCPLFLN20XPH; LWD U PLAN FL PHI OBJ,NA 0.45,WD6.4-7.6MM,W/CC
  • LUCPLFLN40XPH; LWD U PLAN FL PH 2 OBJ,NA0.6,WD2.7-4.0,W/C-COL
  • UPLFLN40XO; U PLAN FLUORITE 40X OIL OBJ,NA 1.30,WD 0.2MM
  • UPLSAPO100XO; U PLAN S-APO 100X OIL OBJ,NA1.40,WD0.12

Fluorescence filter cubes available (8 positions):

  • DAPI-5060C-OFF-ZERO;EX377/50,EM447/60,DM409
  • TXRED-4040C-OFF-ZERO; EX562/40,EM624/40,DM593
  • GFP-3035D-OFF-ZERO; EX472/30,EM520/35,DM495
  • ET Cy5 longpass, EX620/60, EM660lp, DM660lpxrxt
  • ET LI-Cor for IR Dye 800, EX740/40, EM780lp, DM 770lpxr
  • ET DAPI/FITC/Texas Red
  • ET EGFP/mCherry

Olympus BX 50 upright fluorescence microscope

Olympus BX 50 upright fluorescence microscope

Objective available:

  • UPlanFL 10X/0.3
  • UPlanFL 20X/0.5
  • UPlanFL 40X/0.75
  • Zeiss Achroplan 100X/1,25 oil can be placed into the turret on request.

Fluorescence filter cubes:

  • DAPI:ex-BP360-370, DM400,em-BA420
  • FITC:ex-460-490, DM505, em-BA515IF
  • Rhodamine:ex-BP520-550, DM565, em-BA580IF

Zeiss M2Bio Stereomicroscope

Zeiss M2Bio Stereomicroscope

This stereomicroscope system provides low magnification long working distance (stereo) imaging with an 11X zoom. Field widths as large as 2.5 cm can be imaged. The microscope converts to monocular imaging with 10X or 20X LWD objectives and the 11X zoom. Trans or reflected Bright field and fluorescence imaging is possible. Images are recorded with either a B/W or color digital camera using Zeiss Axiovision software.

Objectives available:

  • 0.6X
  • 1.0X
  • 1.6X
  • 10X 0.45 NA f=200
  • 20X 0.6 NA f=200

Fluorescence Cubes available:

  • Q dot 705 ex 460SPUVv2; bs 475DCXRU; em D705/20
  • Coumarin ex D405/10, bs 425DCLP; em D460/50
  • cGFP ex D436/20; bs 455DCLP; em D480/40
  • GFP ex HQ470/40; bs Q495LP; em HQ525/50
  • yGFP ex HQ500/20; bs Q515LP; em HQ535/30
  • TRITC ex D540/25; bs 565DCLP; em D605/55
  • Cy5 ex HQ620/60; bs Q660LP; em HQ700/75

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