Rubin Room 622
Darmouth Cancer Center
One Medical Center Drive
Lebanon, NH 03756
(603) 653-9966
Pricing information and sample submission
We support the generation and utilization of genetically modified mice for members of the Dartmouth research community. We offer a wide variety of services that assist researchers in construct design and creation of novel transgenic mice. We also offer mice with a human immune system that allows our investigators a better way to study the virus and other human infections.
Leadership
Steven Fiering, PhD
(603) 653-9966
Fiering@Dartmouth.edu
Our lab studies the cancer immunology of ovarian and melanoma cancers. We are using treatments with bacteria or nano-particles to decrease or eliminate tumors.
Jennifer Fields, BS
(603) 653-9971
Jennifer.L.Fields@Dartmouth.edu
Jennifer is a veterinary technologist/research assistant. She has extensive skills in creating transgenic mice and performing mouse surgeries. She has been with this core since 1997.
Services
- Transgenic mice: design
- Genetic construct generation: via recombineering in E. coli or yeast
- Generation of transgenic founders: injection of plasmids, BAC or lentivirus into oocytes and transplantion into foster mothers
- Generation of germline chimeric mice: injection of ES cells into blastocysts and transplantion
- ES cell culture: for gene targeting: provide a variety of ES cell lines, transfect with KO constructs, freeze and provide lysates for genotyping, expand targeted clones for mouse generation
- Establish lines: from frozen embryos from other institutions or rederive a pathogen infected transgenic line by embryo transfer
- Cryopreservation: of embryos and sperm to preserve & protect your valuable transgenic mouse lines
- Technical Services: tailvein injections, blood drawing, assist in maintaining transgenic colonies (husbandry and genotyping), timed pregnancy
- Mouse surgery: ovariectomies, vasectomies, other special surgeries, tumor removal, and tissue collection
- Maintaining several transgenic mouse models: FLPe, Cre, VISTA, P53, and NSG
- Tumor induction: using P53 kras or varient mice and Adeno-Cre lenti-virus, we can infect the lung, breast or ovary to create tumors
- Provide consultation on transgenic mice: construct design and production, overall experimental