Genomics Shared Resource

Rubin 6, Bench 63
Dartmouth Cancer Center
One Medical Center Drive
Lebanon, NH 03756
603-646-5502

Geisel School of Medicine Genomics Shared Resource website

Pricing information and sample submission

The Genomics Section provides services and instrumentation that enable DNA/RNA extraction and quality control, next generation sequencing, epigenetic profiling and microarray analysis on a whole-genome scale, from the level organisms to single cells and spatially within tissues. Emphasis is placed on providing Cancer Center investigators access to cutting-edge technologies to generate high-quality genomics data in this rapidly evolving and increasingly utilized field.

Leadership

Fred Kolling, PhD

Fred Kolling, PhD
Director
fred.w.kolling.iv@dartmouth.edu
603-646-5487

Elizabeth Sergison

Elizabeth Sergison
Co-Director
Elizabeth.Ann.Sergison@dartmouth.edu
 

Genomics instrumentation

Fragment Analyzer

  • Capillary electrophoresis instrument for quality control of DNA and RNA samples

Illumina Nextseq2000 and Novaseq6000

  • High throughput Illumina sequencing for bulk and single cell DNA/RNA sequencing projects

10x Genomics Chromium

  • Single cell genomics on fresh and fixed samples

10x Genomics CytAssist

  • Spatial transcriptomics on fresh frozen (FF) and formalin-fixed paraffin embedded (FFPE) specimens
  • Tissue sectioning, staining and imaging performed in collaboration with Pathology Shared Resource

10x Genomics Xenium Analyzer

  • In situ measurement of up to 500 gene targets in fresh or FFPE tissue sections at 50nM resolution

EpMotion 5075t

  • Automated liquid handling for NGS library preparation and sample normalization

Genomics services

Experimental design

  • Assessment of feasibility
  • Selection of appropriate technology
  • Cost evaluation
  • Discussion of data analysis resources

Sample processing and QC

  • DNA/RNA extraction from fresh, frozen, or FFPE cells/tissues
  • Capillary electrophoresis for DNA/RNA quality assessment
  • Qubit DNA/RNA fluorometric quantification
  • Single cell/nuclei isolation for single cell genomics
  • Automated cell counting, fluorescent live/dead staining

Microarrays

  • Illumina Infinium arrays
    • CNV/SNP arrays
    • DNA Methylation

Next-generation sequencing (Illumina and Oxford Nanopore)

  • RNA
    • 3’-End, PolyA or Ribodepletion workflows
    • miRNA/smRNA profiling
  • DNA
    • Whole genome/exome sequencing
    • User-prepared libraries (CutNRun, ChIP-seq, etc.)
  • Plasmid sequencing

Single cell genomics (10x Chromium)

  • 3’/5’ mRNA-seq
  • TCR/BCR-seq
  • ATAC-seq
  • Multiome (RNA+ATAC)
  • Flex (Fixed RNA profiling)