Genomics Shared Resource

Rubin Room 670
Dartmouth Cancer Center
One Medical Center Drive
Lebanon, NH 03756
(603) 653-9978

Geisel School of Medicine Genomics Shared Resource website

Pricing information and sample submission

The Genomics Section provides services and instrumentation that enable DNA/RNA extraction and quality control, next generation sequencing, epigenetic profiling and microarray analysis on a whole-genome scale, from the level organisms to single cells. Emphasis is placed on providing Cancer Center investigators access to cutting edge technologies to generate high quality genomics data in this rapidly evolving and increasingly utilized field.

Leadership

Craig Tomlinson, PhD

Craig R. Tomlinson, PhD
Scientific Director
Craig.R.Tomlinson@Dartmouth.edu
Departments of Medicine and Pharmacology & Toxicology

Fred Kolling, PhD

Fred Kolling, PhD
Co-Director
fred.w.kolling.iv@dartmouth.edu
(603) 653-9978

Heidi Trask, BS

Heidi Trask, BS
Lab Manager
Heidi.W.Trask@Dartmouth.edu
(603) 653-9978

Elizabeth Sergison, PhD

Elizabeth Sergison, PhD
Lead Scientist, Gene Editing
Elizabeth.A.Sergison.GR@Dartmouth.edu

Genomics instrumentation

Fragment Analyzer

  • Capillary electrophoresis instrument for quality control of DNA and RNA samples
  • Input range from <1ng-500ng of DNA/RNA

Illumina NextSeq500 and MiniSeq

  • Used for all Next Generation Sequencing within the Genomics Shared Resource
  • Wide range of sequencing output, from 8 million – 400 million reads per run

10x Genomics Chromium

  • High throughput single cell genomics
    • Single cell RNA-seq, TCR/BCR-seq, DNA-seq and ATAC-seq available

EpMotion 5075t

  • Automated liquid handling solution
    • NGS Libraries
    • 96/384-well plate formats supported
    • Temperature control modules and thermomixer available

Affymetrix Fluidics Station and Gene Chip Scanner

  • Enables Affymetrix RNA gene expression and DNA CNV/SNP arrays

Genomics services

Experimental design

  • Assessment of feasibility
  • Selection of appropriate technology
  • Cost evaluation
  • Discussion of data analysis resources

Microarrays

  • Illumina Infinium arrays
    • CNV/SNP arrays
    • DNA Methylation
  • Affymetrix GeneChip arrays
    • RNA: mRNA, lncRNA and miRNA
    • DNA: CNVs and Cytogenetics

Next-generation sequencing (Illumina)

  • RNA
    • 3’-End, PolyA or Ribodepletion workflows
    • miRNA/smRNA profiling
    • Targeted panels (amplicon sequencing)
  • DNA
    • Whole Genome Sequencing
    • ChIP-seq
    • Targeted panels (probe or amplicon-based)
    • Methyl-seq
    • Linked-read sequencing (10x Genomics)

Single cell genomics (10x Genomics)

  • 3’/5’ mRNA-seq
  • TCR/BCR-seq
  • DNA-seq (CNV and Cytogenetics)
  • ATAC-seq

Quality control for RNA and DNA

  • Qubit 2.0 fluorimeter: specific, accurate quantitation of DNA or RNA
  • NanoDrop: Estimation of DNA/RNA concentration and relative purity
  • Fragment Analyzer (Advanced Analytical): Capillary electrophoresis for DNA/RNA
  • quality assessment