Rubin Room 670
Dartmouth Cancer Center
One Medical Center Drive
Lebanon, NH 03756
(603) 653-9978
Geisel School of Medicine Genomics Shared Resource website
Pricing information and sample submission
The Genomics Section provides services and instrumentation that enable DNA/RNA extraction and quality control, next generation sequencing, epigenetic profiling and microarray analysis on a whole-genome scale, from the level organisms to single cells. Emphasis is placed on providing Cancer Center investigators access to cutting edge technologies to generate high quality genomics data in this rapidly evolving and increasingly utilized field.
Leadership
Craig R. Tomlinson, PhD
Scientific Director
Craig.R.Tomlinson@Dartmouth.edu
Departments of Medicine and Pharmacology & Toxicology
Fred Kolling, PhD
Co-Director
fred.w.kolling.iv@dartmouth.edu
(603) 653-9978
Heidi Trask, BS
Lab Manager
Heidi.W.Trask@Dartmouth.edu
(603) 653-9978
Elizabeth Sergison, PhD
Lead Scientist, Gene Editing
Elizabeth.A.Sergison.GR@Dartmouth.edu
Genomics instrumentation
Fragment Analyzer
- Capillary electrophoresis instrument for quality control of DNA and RNA samples
- Input range from <1ng-500ng of DNA/RNA
Illumina NextSeq500 and MiniSeq
- Used for all Next Generation Sequencing within the Genomics Shared Resource
- Wide range of sequencing output, from 8 million – 400 million reads per run
10x Genomics Chromium
- High throughput single cell genomics
- Single cell RNA-seq, TCR/BCR-seq, DNA-seq and ATAC-seq available
EpMotion 5075t
- Automated liquid handling solution
- NGS Libraries
- 96/384-well plate formats supported
- Temperature control modules and thermomixer available
Affymetrix Fluidics Station and Gene Chip Scanner
- Enables Affymetrix RNA gene expression and DNA CNV/SNP arrays
Genomics services
Experimental design
- Assessment of feasibility
- Selection of appropriate technology
- Cost evaluation
- Discussion of data analysis resources
Microarrays
- Illumina Infinium arrays
- CNV/SNP arrays
- DNA Methylation
- Affymetrix GeneChip arrays
- RNA: mRNA, lncRNA and miRNA
- DNA: CNVs and Cytogenetics
Next-generation sequencing (Illumina)
- RNA
- 3’-End, PolyA or Ribodepletion workflows
- miRNA/smRNA profiling
- Targeted panels (amplicon sequencing)
- DNA
- Whole Genome Sequencing
- ChIP-seq
- Targeted panels (probe or amplicon-based)
- Methyl-seq
- Linked-read sequencing (10x Genomics)
Single cell genomics (10x Genomics)
- 3’/5’ mRNA-seq
- TCR/BCR-seq
- DNA-seq (CNV and Cytogenetics)
- ATAC-seq
Quality control for RNA and DNA
- Qubit 2.0 fluorimeter: specific, accurate quantitation of DNA or RNA
- NanoDrop: Estimation of DNA/RNA concentration and relative purity
- Fragment Analyzer (Advanced Analytical): Capillary electrophoresis for DNA/RNA
- quality assessment