Immune Monitoring and Flow Cytometry Resource

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Immune Monitoring and Flow Cytometry Resource

Immune Monitoring and Flow Cytometry
Dartmouth Cancer Center
Rubin Building 710 (Immune Monitoring)
Borwell Building 342W (Flow Cytometry)
One Medical Center Drive
Lebanon, NH 03756
Email: daniel.w.mielcarz@dartmouth.edu
Hours: Monday through Friday, 8:30 am to 6:00 pm, 24-hour access to flow cytometers

Geisel School of Medicine DartLab website

Pricing information and sample submission

Equipment

Training and consultation

We provide various services such as isolation and cryopreservation of PBMCs and plasma or serum for clinical trials, as well as carry out a spectrum of immunoassays and develop customized immunoassays. We also provide dedicated equipment such as cell sorters, flow cytometers, ELISPOT reader, Bio-Plex array reader, and Isolight single-cell proteomics reader.

Leadership

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Daniel Mielcarz - Leadership Immune Monitoring

Daniel Mielcarz, PhD
Director
Senior Research Scientist

Dr. Mielcarz’s interests lie in monitoring human immunology, particularly in cancer immunotherapy. He is a member of the Federation of Clinical Immunology Societies (FOCIS), The Association of Biomolecular Resource Facilities (ABRF), the International Society for the Advancement of Cytometry (ISAC), and North East Regional Life Science Core Directors (NERLSCD).

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Alan Bergeron, BS

Alan Bergeron, BS
Immune Monitoring Laboratory Manager
Affiliations: The Association of Biomolecular Resource Facilities (ABRF)
North East Regional Life Science Core Directors (NERLSCD)
ajb@dartmouth.edu

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Gary Ward, BS

Gary Ward, BS
Flow Cytometry Laboratory Manager
Affiliations: The Association of Biomolecular Resource Facilities (ABRF)
North East Regional Life Science Core Directors (NERLSCD)
gary.ward@dartmouth.edu

Equipment

Flow Cytometers

  • FACSAria Cell Sorter III (17 color)
  • SH-800 Cell Sorter (6 color)
  • Aurora 5-laser Spectral Cytometer (40 color)
  • ZE-5 Cytometer (27 color)
  • Yeti Cytometer (27 color)
  • Cytoflex Cytometer (13 color)
  • Gallios Cytometer (10 color)
  • Three MACSQuant10 Cytometers (8 color)

Immunoassays

  • Bioplex 200 Luminex System
  • Zeiss Axioplan2 ELISPOT Reader
  • Isolight Single-Cell Proteomics Platform

Services

  • Whole blood processing
  • Custom immunoassays for clinical trials
  • Cell subset enrichment
  • Leukocyte phenotyping
  • ELISPOT assay
  • CFSE lymphoproliferation assay
  • Up to 48-plex cytokine assay
  • FACS (cell sorting)
  • Up to 25 color flow cytometry
  • Flow cytometry analysis
  • Pre-experiment consultation
  • Data analysis assistance

Access

  • Following user training, all flow cytometers (except the Cytoflex) are available for operator-unassisted use by card access 24/7 in Rubin 710. Request training or sign-up using RaDar.
  • The Cytoflex cytometer is available for operator-unassisted use by code access 24/7 in Remsen 243C. Request training and sign-up using RaDar.
  • FACSAria III cell sorting in Rubin 710 is available 9:00 am to 5:00 pm. Pre-arrange with Gary Ward. Cytokine assays are pre-arranged with Andrew Calkins in Rubin 710.
  • All other immunoassays are pre-arranged with Alan Bergeron after discussion with Dan Mielcarz.

Turnaround time

  • Flow cytometry and cell-sorting: Acquisition files available same day
  • Multiplexed cytokine assays: Analyzed results next day
  • Whole blood processing: Same day
  • ELISPOT assays: Analyzed results next day
  • Other immunoassays: As arranged

Training and consultation

Flow Cytometry course (5 lectures)

  • Instrument training
  • Consultation for experimental design, multi-color antibody cocktail work-up, and flow cytometry analysis.

Data analysis and interpretation

Computers are available in Rubin 721 for flow cytometry analysis using the latest FlowJo and Graphpad Prism software. Core personnel are available to discuss experiments and to help with data analysis and interpretation. You are strongly encouraged to discuss multi-color flow cytometry experiments with Dan Mielcarz BEFOREHAND.