DartLab: Immune Monitoring and Flow Cytometry
Dartmouth Cancer Center
Rubin Building 710 (Satellite facility: Remsen 243)
One Medical Center Drive
Lebanon, NH 03756
Email: daniel.w.mielcarz@dartmouth.edu
Hours: Monday through Friday, 8:30 am to 6 pm, 24-hour access to flow cytometers
Refer to the following websites for more information:
We provide various services, including the isolation and cryopreservation of PBMCs and plasma or serum for clinical trials, as well as a spectrum of immunoassays, such as flow cytometry and multiplexed cytokine analysis. We also maintain and provide dedicated equipment such as cell sorters, flow cytometers, ELISPOT reader, Bio-Plex array reader, and Isolight single-cell proteomics reader.
On this page:
Leadership
Daniel Mielcarz, PhD
Director
Senior Research Scientist
daniel.w.mielcarz@dartmouth.edu
Dr. Mielcarz's interests lie in monitoring human immunology, particularly in cancer immunotherapy. He is a member of the Federation of Clinical Immunology Societies (FOCIS), the Association of Biomolecular Resource Facilities (ABRF), the International Society for the Advancement of Cytometry (ISAC), and Northeast Regional Laboratory Staff and Core Directors (NERLSCD).
Gary Ward, BS
Flow Cytometry Laboratory Manager
Affiliations: The Association of Biomolecular Resource Facilities (ABRF) and Northeast Regional Laboratory Staff and Core Directors (NERLSCD)
gary.ward@dartmouth.edu
Equipment
Flow Cytometers
- FACSAria Fusion Cell Sorter (18 color)
- SH-800 Cell Sorter (6 color)
- Two (2) Aurora 5-laser Spectral Cytometers (40 color)
- ZE-5 Cytometer (27 color)
- Yeti Cytometer (27 color)
- Cytoflex Cytometer (13 color)
- Three (3) MACSQuant10 Cytometers (8 color)
Immunoassays
- Bioplex 200 Luminex System
- Zeiss Axioplan2 ELISPOT Reader
- Isolight Single-Cell Proteomics Platform
Services
- Whole blood processing
- Custom immunoassays for clinical trials
- Cell subset enrichment
- Leukocyte phenotyping
- ELISPOT assay
- CFSE lymphoproliferation assay
- Up to 48-plex cytokine assay
- ELISA assay
- FACS (cell sorting)
- Up to 25 color flow cytometry
- Flow cytometry analysis
- Pre-experiment consultation
- Data analysis assistance
Access
- Following user training, all flow cytometers are available for operator-unassisted use by card access 24/7 in Rubin 710. Request training or sign-up using RaDar.
- The Cytoflex and Yeti cytometers are available for operator-unassisted use by code access 24/7 in Remsen 243C. Request training and sign-up using RaDar.
- FACSAria Fusion cell sorting in Rubin 710 is available 9 am to 5 pm. Pre-arrange with Gary Ward.
- Multiplexed cytokine assays are pre-arranged with Andrew Calkins in Rubin 710.
- All other immunoassays are pre-arranged with Alan Bergeron after discussion with Dan Mielcarz.
Turnaround time
- Flow cytometry and cell-sorting: Acquisition files available same day
- Multiplexed cytokine assays: Analyzed results next day
- Whole blood processing: Same day
- ELISPOT assays: Analyzed results next day
- Other immunoassays: As arranged
Training and consultation
Flow Cytometry course (5 lectures)
- Instrument training
- Consultation for experimental design, multi-color antibody cocktail work-up, and flow cytometry analysis
Data analysis and interpretation
Computers are available in Rubin 721 for flow cytometry analysis using the latest FlowJo and Graphpad Prism software. Core personnel are available to discuss experiments and to help with data analysis and interpretation. You are strongly encouraged to discuss multi-color flow cytometry experiments with Dan Mielcarz before beginning a study.