Immune Monitoring and Flow Cytometry Resource

Image
Immune Monitoring and Flow Cytometry Resource

Immune Monitoring and Flow Cytometry
Norris Cotton Cancer Center
Rubin Building 710 (Immune Monitoring)
Borwell Building 342W (Flow Cytometry)
One Medical Center Drive
Lebanon, NH 03756
Email: Jacqueline.Smith@Dartmouth.edu
Hours: Monday through Friday, 8:30 am to 6:00 pm, 24-hour access to flow cytometers

Geisel School of Medicine DartLab website

Pricing information and sample submission

Equipment

Training and consultation

We provide various services such as isolation and cryopreservation of PBMCs and plasma or serum for clinical trials, as well as carrying out a spectrum of immunoassays and developing customized immunoassays. It also provides dedicated equipment such as cell sorters, flow cytometers, ELISPOT reader, Bio-Plex array reader, autoMACS and RoboSep.

Leadership

Image
Jacqueline Channon Smith, PhD

Jacqueline Channon Smith, PhD
Faculty Director
Research Assistant
Professor, Department of Microbiology and Immunology

Dr. Smith's interests lie in monitoring human immunology, particularly multiple sclerosis. She is a member of the Federation of Clinical Immunology Societies (FOCIS), International Clinical Cytometry Society (ICCS), and International Society for the Advancement of Cytometry (ISAC).

Image
Alan Bergeron, BS

Alan Bergeron, BS
Immune Monitoring Laboratory Manager
Affiliations: The Association of Biomolecular Resource Facilities (ABRF)
North East Regional Life Science Core Directors (NERLSCD)
ajb@Dartmouth.edu

Image
Gary Ward, BS

Gary Ward, BS
Flow Cytometry Laboratory Manager
Affiliations: The Association of Biomolecular Resource Facilities (ABRF)
North East Regional Life Science Core Directors (NERLSCD)
Gary.Ward@Dartmouth.edu

Equipment

Flow Cytometers

  • FACSAria Cell Sorter (11 color)
  • Gallios Cytometer (10 color)
  • MACSQuant10 (8 color)
  • MacsQuantVYB (8 color)
  • MacsQuantAnalyzer (7 color)
  • FACSCanto (5 color)
  • FACSCalibur (4 color)
  • FACScan (3 color)

Immunoassays

  • Bioplex 200 Luminex System
  • MSD Sector Imager
  • Zeiss Axioplan2 ELISPOT Reader
  • StemCell Robosep
  • AutoMACS

Services

  • Whole blood processing
  • Custom immunoassays for clinical trials
  • Cell subset enrichment
  • Leukocyte phenotyping
  • ELISPOT assay
  • CFSE lymphoproliferation assay
  • Up to 42-plex cytokine assay
  • FACS (cell sorting)
  • Up to 10-color flow cytometry
  • Flow cytometry analysis
  • Pre-experiment consultation
  • Data analysis assistance

Access

  • FACScan, FACSCalibur and FACSCanto cytometers are available for operator-unassisted use by card access 24/7 in Borwell 342W - sign-up using CCOPS.  
  • MACSQuant and Gallios cytometers are available for operator-assisted use in Rubin 710 and Remsen 5 from 9 am through 6 pm – prearrange with Alan Bergeron.
  • FACSAria cell sorting in Borwell 336W is available 9 am through 5 pm – pre-arrange with Gary Ward.  
  • Cytokine assays are pre-arranged with Kathy Smith in Rubin 710.
  • All other immunoassays are pre-arranged with Alan Bergeron after discussion with Jacqueline Smith.
  • Computers are available in Borwell 340W for flow cytometry analysis using the latest FlowJo, Modfit, Gemstone, and Graphpad Prism software. Core personnel are available to discuss experiments and to help with data analysis and interpretation. You are strongly encouraged to discuss multi-color flow cytometry experiments with Jacqueline Smith or Dan Mielcarz before you begin.

Turnaround time

  • Flow cytometry and cell-sorting – acquisition files available same day
  • Multiplexed cytokine assays – analyzed results next day
  • Whole blood processing – same day
  • ELISPOT assays – analyzed results next day
  • Other immunoassays – as arranged

Training and consultation

Flow Cytometry course (5 lectures)

  • Instrument training
  • Consultation for experimental design, multi-color antibody cocktail work-up, and flow cytometry analysis.
  • FACScan, FACSCalibur, MACSQuant, and Gallios cytometers are available for operator-unassisted use by card access 24/7 in Borwell 342W. Sign-up using CCOPS.
  • MACSQuant10 and Gallios cytometers are available for operator-assisted use in Rubin 710 and Borwell 324W from 9 am through 6 pm – pre-arrange with Alan Bergeron.
  • MACSQuant VYB and FACSCanto cytometers are available for operator-unassisted use in Remsen 516 with 24/7 access.
  • FACSAria cell sorting in Borwell 336W is available 9 am through 5 pm – pre-arrange with Gary Ward.
  • Cytokine assays are pre-arranged with Alan Bergeron in Rubin 710.
  • All other immunoassays are pre-arranged with Alan Bergeron after consultation with Jacqueline Smith.

Data analysis and interpretation

Computers are available in Borwell 340W for flow cytometry analysis using the latest FlowJo, Modfit, Gemstone, and Graphpad Prism software. Core personnel are available to discuss experiments and to help with data analysis and interpretation. You are strongly encouraged to discuss multi-color flow cytometry experiments with Jacqueline Smith or Dan Mielcarz BEFOREHAND.