Microscopy Resource

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Microscopy Resource

Borwell 338 West
Norris Cotton Cancer Center
One Medical Center Drive
Lebanon, NH 03756
(603) 650-7661
Hours: M-F 9 am-5:30 pm, authorized users have 24/7 access.

Overview

The NCCC Microscopy Shared Resource provides access to advanced imaging instrumentation and image analysis software to all members of Dartmouth College and Dartmouth-Hitchcock communities. The instrumentation available include point scanning confocal microscopy and Airyscan super resolution, multichannel total internal reflection fluorescence microscopy (TIRFM), automated multi-spectral image acquisition and spectral unmixing, as well as more conventional modalities such as widefield fluorescence and brightfield imaging. To enable effective and safe use of the MSR resources, facility personnel are available for training of prospective users in image theory and hands-on use of specific instrumentation. Facility personnel are available for consultation on experimental design, data acquisition, and analysis as well as to perform data acquisition and analysis for interested labs.

Leadership

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Radu V. Stan, MD, PhD

Radu V. Stan, MD, PhD
Faculty Director
Associate Professor
Departments of Biochemistry and Cell Biology, and of Pathology
Rubn 623
Phone: (603) 650-8781
Fax: (603) 653-9952
Radu.V.Stan@Dartmouth.edu

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Pat Robison

Pat Robison, PhD
Manager
Borwell 383 West (IPIM)
Remsen 611 (MIIC)
(603) 650-7661
Pat.Robison@Dartmouth.edu

Training

Facility staff provide an introductory imaging theory lecture set several times per year. Users who have completed this training may request hands-on training sessions to qualify for independent use. Please contact Pat Robison (facility manager) with a completed training request form (DOC) to schedule training.

Services

We provide consultation services for imaging experiments at no cost to users. Facility personnel are available to work with investigators using the microscopes at an additional hourly cost or to perform data acquisition and image analysis on a fee for service basis. Please contact Pat Robison (facility manager) to access these services.

Usage

Authorized users may access microscope calendars through RaDar.

Only fully trained and authorized users will be able to see the microscope schedules. Please note that hours have been restricted to accommodate Covid-19 protocols. Due to these protocols we require that imaging or image analysis sessions to be booked through RaDar 24 hours in advance. In addition to reserving instrumentation on RaDar, users must record their physical presence in the facility on the Borwell 338 Contact Tracing Calendar (e-mail Pat Robison for access).

Instrumentation

Zeiss LSM 800 with Airyscan

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LSM800 Scanner
This microscope features classical confocal point scanning capability for optical sectioning and 3D reconstruction as well as sub-diffraction resolution capability through the Airyscan detector. Adjustable spectral filters and 4 lasers make this a suitable system for almost any dye in the visible range. An environmental chamber with temperatures, humidity, and gas mixture control allows extended observation of live samples.

 

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PerkinElmer Vectra® 3

PerkinElmer Vectra3 automated pathology imaging system

This system is an automated slide scanner for scanning of up to Designed to six slides with H&E, IHC, or fluorescent staining. The instrumentation is capable of multi-spectral imaging and spectral unmixing, which allows for a high degree of multiplexing of a broad range of fluorophores in the same sample. Vectra, Phenochart, inForm and HALO3 software is available for acquisition control and image analysis.

 

 

 

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IncuCyte® ZOOM

Essen BioScience Incucyte ZOOM

This system resides full-time inside a CO2 cell culture incubator and can be used for imaging of up to 6 multi well cell culture plates with enhanced phase contrast or two-channel florescence. It is ideal for performing high throughput long-term experiments on live samples without disrupting culture conditions.

 

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Olympus IX-73 Inverted Fluorescence Microscope with Olympus DP73 computer controlled CCD camera

Olympus IX83 ZDC2 CellTIRF Inverted Fluorescence Microscope

This microscope is equipped for live-cell, time-lapse brightfield/DIC and 4-channel internal reflection (TIRFm) and widefield fluorescence microscopy. The instrument has a CellTIRF illuminator allowing 4 channel TIRF imaging at equal penetration depth. A TuCam beam splitter and two Andor Zyla sCMOS cameras allow simultaneous two channel imaging in either TIRFM or widefield.

Analysis Workstation

We offer an independent workstation for post-acquisition analysis featuring multiple image analysis packages including HALO3,InForm and Volocity.